DMEM, high glucose, Fetal Bovine Serum- FBS, L- Glutamine- 200 mM, Penicillin- Streptomycin- 10, 000 U mL, 2- Mercaptoethanol, Non- Essential Amino Acids Solution- 100X, Sodium Pyruvate- 100 mM-, Lipofectamine CRISPRMAX Cas9 Transfection Reagent, Distilled Water- molecular grade, Lipofectamine LTX Reagent with PLUS Reagent, PBS pH 7. 4

Hyderabad, Telangana, India