Probe qPCR premix 2X with hot start PCR enzyme with an antiTaq antibody suitable for Taqman probe based real time qPCR- 5 ml or equivalent, Hot start 2X PCR master mix with loading dye added for high- yield conventional PCR- 800 reactions of 50 microlitres each or equivalent, Spin column based gel extraction kit suitable for purification of DNA from excised agarose gel- 250 columns per kit, 2X Master Mix or kit for Taqman probe based Real- Time RT- qPCR- 5 ml or equivalent, Recombinant DNase I- Should be RNase- free- 1000 units, Proteinase K solution or lyophilized- 5 ml of 20 mg per ml or equivalent, Nuclease free ultrapure water- 500 ml, One step RT- PCR kit- 100 reactions of 50 microlit each or equivalent